CHIP在人颅内动脉瘤瘤壁的表达及意义

The Expression and Role of CHIP on Intracranial Aneurysm Wall

目的:观察Hsc70羧基末端反应蛋白(CHIP)在人颅内动脉瘤内的表达情况,检测其对瘤壁血管平滑肌细胞凋亡的作用,揭示CHIP与颅内动脉瘤形成的关系。方法:选择2009年6月至2012年12月在包头医学院第一附属医院显微手术切除的颅内动脉瘤标本18例为研究对象,取脑外伤后内减压术切除的正常脑皮层动脉血管5例作为对照,应用免疫印迹的方法检测标本中CHIP和半胱氨酸天冬酶-3(Caspase-3)的表达;通过RT-PCR的方法检测标本内的CHIP mRNA表达。结果:动脉瘤组CHIP的蛋白表达水平低于对照组,Caspase-3的蛋白表达高于对照组;CHIP mRNA在颅内动脉瘤的表达水平低于对照组,差异均有统计学意义。结论:CHIP可能与颅内动脉瘤的形成与生长有关。

Objective： To detect the expression of Carboxyl terminus of Hsc70 - interacting protein （CHIP） on the intracra- nial aneurysm wall and to assess its effect on the apoptosis of vascular smooth muscle cells, aiming at clarifying the relationship between CHIP and the development of intracranial aneurysm. Methods： 18 intracranial aneurysm samples, removed by microsur- gery in the hospital from June 2009 to December 2012, were collected as the experimental group and 5 cases of normal removed pallium artery samples were collected as the control group. Western blotting technique was used to evaluate the expressions of CHIP and caspase - 3. The expression of CHIP mRNA was evaluated by RT - PCR. Results ： Compared with the normal group, the expression of CHIP significantly decreased on the intracranial aneurysm walls while the expression of caspase - 3 remarkably increased （ P 〈 0.01 ）. Moreover, the expression of CHIP mRNA in intracranial aneurysm samples was lower in the experimen- tal group than that in the control group （ P 〈0.01 ）. Conclusion： CHIP may be involved in the formation and the progression of intracranial aneurysm.