不同粒径二氧化硅粉尘对大鼠肺组织中LIGHT基因表达影响的比较研究

A Comparative Study of the Influence of Different Size Silica on LIGHT Expression in the Lungs of Rats

目的:观察微米级二氧化硅(μm-SiO2)和纳米级二氧化硅(nm-SiO2)粉尘对大鼠肺组织中LIGHT基因表达水平的影响。方法:56只雄性Wistar大鼠随机分7组,即nm-SiO2、μm-SiO2各分设10 mg/100 g、15 mg/100 g、20 mg/100 g 3个剂量组和1个对照组;采用气管滴注法对实验组滴注相应剂量的二氧化硅悬液,对照组滴注生理盐水,1次/d,连续2周;造模结束后左心室放血处死,利用RT-PCR方法研究染毒大鼠肺组织中LIGHT基因表达水平的变化。结果:微米实验组大鼠肺组织中LIGHT基因的表达水平增高,与对照组比较差异有统计学意义(P<0.05);纳米实验组大鼠肺组织中LIGHT基因的表达水平增高,与对照组比较差异有统计学意义(P<0.05),且高于微米组(P<0.05)。结论:炎症早期,LIGHT基因在μm-SiO2和nm-SiO2实验组中表达均增高;二氧化硅粒径越小,LIGHT基因的表达量越高。

Objective ： To observe the influence of different size individual silica dust on LIGHT gene expression in the lung tissues of rats. Methods： 56 Wistar rats were randomly divided into seven groups, 3 groups of nm - SiO2 （ 10 mg/100 g, 15 mg/100 g, 20 mg/100 g）, 3 groups ofμm - SiO2 （ 10 mg/100 g, 15 mg/100 g, 20 mg/100 g） and a control group. SiO2 suspension was transfused into the rats from different dose groups by the trachea transfusion and the salt water for those in the con- trol group, once a day, for two weeks, to form the silica - affected model. The modelled rats were killed with bleeding in the left ventricle of heart and the changes in LIGHT gene expression were observed in the lung tissues of the silica - affected rats by RT - PCR. Results ： compared with the controls, LIGHT gene expression level was raised in the lung tissues of the rats inl^m - SiO2 groups （ P 〈 0.05 ）. Compared with the controls, LIGHT gene expression level was raised as well in the lung tissues of the rats in nm - SiO2 groups, even higher than that in Ixm - SiO2 groups （ P 〈 0.05 ）. Conclusion ： LIGHT gene expression level was raised in both μm - SiO2 and nm - SiO2 groups in early inflammation. The smaller the silica particle size is, the higher LIGHT gene expression level will be.