农用化学品污染对土壤微生物群落DNA序列多样性影响研究

A preliminary study on DNA sequence diversity of soil microbial community affected by agricultural chemicals

采用 RAPD分子遗传标记技术研究了农用化学品不同使用环境下的 4种土壤微生物群落 DNA序列多样性的变化。结果表明 ,4种土壤微生物群落 DNA序列在其丰富度、多样性指数、均匀度等方面均存在差异 ;农用化学品的使用会对土壤微生物群落在 DNA分子水平上的多样性产生影响 ;而不同的农用化学品对土壤微生物群落 DNA序列多样性影响各不相同 :化肥污染会引起某些土壤微生物的富集和一些微生物物种的丧失 ;农药污染会引起土壤微生物 DNA序列本身发生变化。

The DNA sequence diversity in four soil microbial communities affected by agricultural chemicals was evaluated by using random amplified polymorphic DNA(RAPD)fingerprints.The results showed that these soil microbial communities had differences at DNA sequence richness,diversity index and evenness.The changes in the soil microbial community diversity by agricultural chemicals were found at the DNA level.It was suggested that enrichment or loss of some microbial species may occur in the soils polluted by fertilizers and that DNA sequences of microbial communities may be changed in the soils polluted by pesticides.