蒽对 3种海洋微藻致毒效应的研究

TOXIC EFFECTS OF ANTHRACENE ON THREE SPECIES OF MARINE MICROALGAE

新月菱形藻 (N itzschia closterium)、绿光等鞭金藻 870 1(Isochrysis galbana870 1)和亚心形扁藻 (Platy-monas subcordiformis) 3种海洋微藻用不同浓度的蒽胁迫 72 h,分别测定 3种藻的生长速率、光合色素含量、净光合速率及呼吸速率的变化。结果表明 :3种藻对蒽的敏感性由高到低依次为 :新月菱形藻、金藻 870 1和亚心形扁藻 ;3种微藻呼吸作用受抑制的程度要高于光合作用 ;光合色素含量的变化趋势与光合作用的变化趋势呈一定的正相关 ;但有时光合色素含量低而光合作用强 ;比较 3种藻的净呼吸作用速率发现 ,新月菱形藻呼吸作用相对较强。

Three species of microalgae were exposed to toxic stress from a range of concentrations of anthracine for a period of 72 hours. Following exposure the following measures of stress were recorded: the rates of production of photosynthetic pigments, photosynthesis and respiration. Sensitivity to this stress varied between species, which were, in declining order of sensitivity Nitzschia clostertum, Isochrysis galbana 8701 and Platymonas subcordiformis , with 72 h·EC 50 of 0.06 mg·L -1 , 0.065 mg·L -1 and 0.094 mg·L -1 respectively. Under anthracene stress respiration was more significantly restrained in all three species than was photosynthesis. The pattern of photosynthetic pigment (Chl.a) production was similar to that of photosynthesis, though strong photosynthesis was also occasionally observed with low photosynthetic pigments concentration. Of the three microalgae Nitzschia clostertum had the strongest respiration.