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穿心莲内酯对人黑色素瘤细胞A375凋亡的影响 被引量：5

Influence of andrographolide on apoptosis in human melanoma A375 cell line
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摘要 目的研究穿心莲内酯对人的黑色素瘤细胞(A375)凋亡的影响。方法通过MTT方法检测穿心莲内酯对A375细胞增殖的抑制作用,并求出其半数抑制质量浓度(IC50),并以IC50的穿心莲内酯和衍生物4H-穿心莲内酯作用于A375细胞,并设定溶剂DMSO对照组,Western Blot检测人原癌基因bcl-6的表达,同时用Honechst 33342染液检测A375细胞的凋亡情况。结果穿心莲内酯抑制A375生长的半数抑制质量浓度为30μg/mL;Western Blot检测显示穿心莲内酯明显下调bcl-6的表达;Honechst 33342染色显示穿心莲内酯明显促进了A375细胞的凋亡。结论穿心莲内酯通过下调bcl-6促进A375细胞的凋亡。 AIM To investigate the role of andrographolide on apoptosis in human melanoma cells line A375.METHODS Inhibitory effect of andrographolide on the proliferation of A375 cell line was detected by MTT.IC50 value of andrographolide and 4H-andrographolide was calculated.The solvent DMSO was selected as control group.The expression of bcl-6 was detected by Western Blot and Honechst 33342 dye.RESULTS The IC50 vaule of andrographolide detected by MTT was 30 μg/mL.Andrographolide significantly lowered the expression of bcl-6 by western blot.Honechst 33342 staining showed that andrographolide significantly promoted the apoptosis of A375 cell line.CONCLUSION Andrographolide can promote apoptosis in human melanoma A375 cell line by decreasing bcl-6 level.
作者 方海燕 吴晓云 兰天 杨毅 王丽京 李卫东
机构地区 广东药学院血管生物学研究所
出处 《中成药》 CAS CSCD 北大核心 2013年第4期643-646,共4页 Chinese Traditional Patent Medicine
基金 国家科技部"973"项目(国家重点基础研究发展计划2010CB529702) 国家自然科学基金资助项目(30900764 21005022)
关键词 穿心莲内酯 BCL-6 凋亡 andrographolide bcl-6 apoptosis
分类号 R285.5 [医药卫生—中药学]
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参考文献17

  • 1Poolsup N, Suthisisang C, Prathanturarug S, et al. Androgra- phis paniculata in the symptomatic treatment of uncomplicated upper respiratory tract infection: systematic review of random- ized controlled trials [ J ]. J Clin Pharrn Ther, 2004, 29 ( 1 ) : 37-45.
  • 2Iruretagoyena M I, Tobar J A, Gonzalez P A, et al. Androgra- pholide interferes with T eell activation and reduces experimental autoimmune encephalomyelitis in the mouse [ J ]. J Pharmacol Exp Ther, 2005, 312(1): 366-72.
  • 3Pratheeshkumar P, Sheeja K, Kuttan G. Andrographolide in- duces apoptosis in B16F-10 melanoma ceils by inhibiting NF- KB-mediated bcl-2 activation and modulating p53-induced caspase-3 gene expression[ J ]. Immunopharmacol Immunotoxi- col, 2012, 34(1): 143-51.
  • 4Tsao H, Atkins M B, Sober A J. Management of cutaneous melanoma[J]. N Engl J Med, 2004, 351(10) : 998-1012.
  • 5Charles M, Balch M D, Marshall M. Efficacy of 2-cm surgical margins for intermediate thickness melanomas ( 1 to 4 mm) re- suits of a multi-institutional randomized surgical trial [ J ]. Ann Sur, 1993, 218(3): 262-269.
  • 6Xia Y F, Ye B Q, Li Y D, et al. Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through co- valent modification of reduced cysteine 62 of p50[ J]. J Immu- nol, 2004, 173(6) : 4207-4217.
  • 7Kim T G, Hwi K K, Hung C S. Morphological and biochemical changes of androo'apholide-induced cell death in human prostatic adenocarcinoma PC-3 ceils [ J ]. In Vivo, 2005, 19 (3) :551-557.
  • 8Zhou J, Zhang S, Ong C N, et al. Critical role of pro-apop- totic Bcl-2 family members in andrographolide-induced apoptosis in human cancer cells [ J ]. Biochem Pharmacol, 2006, 72 : 132-144.
  • 9Geethangili M, Rao Y K, Fang S H, et al. Cytotoxic constitu- ents from Andrographis paniculata induce cell cycle arrest in Jurkat cells[J]. Phytother Res, 2008, 22(10) : 1336-1341.
  • 10Shi M D, Lin H H, Lee Y C, et al. Inhibition of cell-cycle pro- gression in human colorectal carcinoma Lovo cells by androgra- pholide[J].Chem Biol Interact, 2008, 174(3) : 201-210.

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中成药
2013年 第4期

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