摘要
目的 研究中国群体 Y染色体微卫星位点 DYS390遗传多态性 ,可以用于追溯人类进化上的父系祖先 ,也可以为人类基因组和法医学等研究积累数据。方法 采用 PCR技术扩增微卫星DNA片段 ,再经变性凝胶电泳及银染方法 ,对我国东北地区汉族、蒙古族及朝鲜族 3个群体的1 0 2例男性个体的 DYS390位点的遗传多态性进行了研究。结果 除汉族群体发现 5种等位基因外 ,朝鲜族和蒙古族群体均检出 4种。在汉族群体中 ,我们检出 1例具 2 2 3bp等位基因。等位基因频率分布在汉族、朝鲜族以 2 1 1 bp的频率为最高 ,分别为 0 .439和 0 .451 ;而蒙古族群体则以 2 1 5bp的频率为最高 ( 0 .433)。结论 3个群体之间 DYS390位点等位基因频率无显著性差异 ( Fisher精确概率检验 :P=0 .930 )。 3个群体中 DYS390位点 5种等位基因的分化程度以 2 2 3bp为最高 ,分化程度最低的为 2 1 1 bp。聚类分析表明 3个群体的父系亲缘关系较为密切 ,其中以汉族与朝鲜族之间的遗传距离最近。
Purpose\ The study of Chinese Y chromosomal microsatellite locus DYS390 are used to trace paternal lineage of human origins and evolution.The data in this study may be useful in human population genetics and forensic applications.Methods\ In this experiment,the distribution of a Y\|chromosome specific polymorphic locus,DYS390,in 102 unrelated males of three ethnic groups in China was studied by using PCR method followed by PAGE and silver staining.The three nationalities are Han in Northeast of our country,Korean in Yan Bian and Mongol in Inner Mongolia Municipalities.Results\ The results showed that 5 alleles were existed in Han population,4 alleles were existed in Korean and Mongolian populations.Their highest allele frequencies were 0 439(211bp) in Han,0 451(211bp)in Korean,and 0 433(215bp) in Mongolian population.Besides,one case of allele 223bp was found in Han population (1/41).Conclusion\ The allele frequency difference among three populations is insignificant (Fisher's Exact Test:p=0 930).Clustering analyses showed that paternal lineage is intimate among the three nationalities.
出处
《人类学学报》
CSCD
北大核心
2000年第4期318-323,共6页
Acta Anthropologica Sinica
基金
国家自然科学基金重大项目资助!课题号 :3 93 92 90 0
