摘要
利它素粗提液或涂利它素粗提液的人造卵经不同温度 (2 5℃、 6 0℃或 10 0℃ )处理 30min后进行生物活性测定 ,发现各温度下利它素仍对野蚕黑卵蜂有很强的引诱活性 ,其平均反应级数与常温 (2 5℃ )相比无明显差异 ,表现出一般蛋白质所没有的热稳定特性。低温 (4℃、 0℃、- 2 0℃或 - 70℃ )同样也能保存粗提液中利它素的生物活性 ,但发现能引起粗提液发生凝集 ,且0℃以下比 4℃能产生更多的凝集 ,使粗提液中利它素活性组分含量减少 ,- 2 0℃时 ,其活性组分的峰面积仅为对照的 13 1% ,表现出一般蛋白质所没有的低温敏感性 ,这一发现对利它素的快速、简捷、有效分离具有重要的指导意义。
Kairomone activities of crude extract from female accessory glands of Bombyx mori after being treated for 30 min at 25℃, 60℃ and 100℃ were determined. The results showed that the extracted substance had strong attractive activity to Telenomus theophilae for all the 3 temperature treatments. The main grade of response at 60℃ or 100℃ showed no significant different from that at 25℃. This meant that the active substance was resistant to high temperature. At low temperature (4℃,0℃,-20℃ or -70℃ ) the substance kept active for eliciting T. theophilae, but these temperatures could cause deposition of the active component. The amount of deposit at 0℃ was larger than that at 4℃. At -20℃ the active component amounted to 13.1% of the control according to HPLC analysis. This finding of sensitivity to low temperature is very importance for simplifying methods for isolation and purification of the kairomone.
出处
《昆虫学报》
CAS
CSCD
北大核心
2000年第4期373-379,共7页
Acta Entomologica Sinica
基金
国家自然科学基金资助项目!(390 70 5 89)
浙江省自然科学基金资助项目!(3982 5 5 )
