丛枝菌根的双重培养方法及其菌丝际的建立

ESTABLISHMENT OF DUAL CULTURE FOR ARBUSCULAR MYCORRHIZA AND FORMATION OF HYPHOSPHERE

成功地建立起丛枝菌根真菌Glomus intraradices与转移Ri T-DNA胡萝卜根器官的双重培养体系，且在此基础上将根与菌丝分隔开来(两室系统)，在人工培养条件下形成了无杂菌的菌丝际环境。菌丝际的建立为研究菌丝的生理、生化特性奠定了基础。

The method of dual culture between Glomus intraradices and transformed Ri T-DNA carrot roots has been established. Based on this method the pure hyphosphere has been formed by separating the roots and hyphae axenically.