猪苓菌丝细胞活性氧的产生及其种类

GENERATION AND VARIETY OF ACTIVE OXYGEN SPECIES IN THE HYPHAE OF GRIFOLA UMBELLATA

用液体发酵的蜜环菌菌丝、菌丝细胞壁及发酵液作为激发子，分别处理猪苓菌丝，均可诱导猪苓菌丝活性氧的产生。活性氧产生量与激发子浓度具相关性。超氧化物歧化酶（SOD）、过氧化氢酶(CAT)、甘露醇均可在一定程度上抑制活性氧的产生，证明活性氧种类包括过氧化氢（H2O2）、羟基自由基（·OH）和超氧根阴离子(O·－2)。Diphenylene iodonium (DPI)能削弱激发子对活性氧的诱导，表明O·－2来源于NADPH氧化酶。

Fermented solution, mycelial extract and cell wall of Armillaria mellea can induce the generation of active oxygen species in Grifola umbellata. The elicitor induced the formation of active oxygen species in a dose-dependent manner. SOD, CAT and mannitol could suppress the production of active oxygen species. It showed active oxygen species included H2O2, O·－2 and ·OH. DPI inhibited active oxygen species induced by elicitors, indicating that NADPH oxidase might be the source of superoxide anion.