HPLC法测定新藤黄酸囊泡溶液中药物的含量及包封率

Determination of neo-gambogic acid content and envelope rate in neo-gambogic acid vesicles solution by HPLC

目的建立测定新藤黄酸囊泡中新藤黄酸含量和包封率的HPLC法。方法用乙醇注入法制备新藤黄酸囊泡,HPLC法测定其中药物含量,微柱离心法测定新藤黄酸囊泡的包封率,其中色谱柱为COSMOSIL C18柱(4.6 mm×250 mm,5μm),流动相为甲醇∶0.1%磷酸(90∶10),进样量20μl,流速1.0 ml/min,检测波长360 nm,温度30℃。结果在上述色谱条件下,辅料和试剂对药物的测定无干扰,分离效果良好。新藤黄酸浓度在0.02～32.00μg/ml范围内线性关系良好(r=0.9996),平均回收率为99.27%,RSD为1.03%(n=3)。新藤黄酸囊泡的包封率为(48.0±1.0)%。结论所建立的HPLC法准确、可靠、重复性好,可用于新藤黄酸囊泡中新藤黄酸含量及包封率的测定。

Objective To establish HPLC method to determine the content and envelope rate of neo - gambogic acid in neo - gambogic acid vesicles solution. Methods The ethanol - injection method was carried out to prepare the neo - gambogic acid vesicles. The chromatographic column was Cosmosil C18 （4.6mm×250mm,5μm） ;the flow phase was methanol - 0.1% orthophosphoric acid （90：10） ; the sample size was 20 p.1; the flow rate was 1.0 ml/min; the detecting wavelength was 360 nm; and the temperature was 30 ℃. Results Under those chromatographic conditions above, adjuvants and reagent had no intervention on the detection of the medicine, and the isolation effect was good. Gambogic acid had a good liner relation at the content range of 0.02 - 32.00 μg/ml （r = 0. 9996,n = 7）. The average recovery rate was 99.27% , and RSD was 1.03% （ n = 3 ）. The envelope rate was （48.0 ± 1.0） %. Conclusion This HPLC method is accurate,reliable, and repeatable and can be used in the detection of neo - gambogic acid content and envelope rate in neo - gambogic acid vesicles solution.