南方四季杨组培再生体系的建立

Establishment of the Tissue Culture Regeneration System of Populus deltoides×P.nigra cv.Chile

以南方四季杨幼嫩叶片和茎段为外植体进行组织培养,探讨基本培养基、生长素、细胞分裂素等因素对其离体再生的影响,结果表明:叶片不定芽分化最佳培养基为MS+6-BA 0.25 mg/L+NAA 0.09 mg/L+琼脂5 g/L+蔗糖30 g/L,分化率可达99.07%;茎段诱导愈伤组织最佳培养基为1/2MS+6-BA 0.1 mg/L+NAA 0.1 mg/L+IAA 0.1 mg/L+琼脂5 g/L+蔗糖30 g/L,诱导所得的愈伤组织在分化培养基上可以再分化出大量再生芽;用1/2 MS+IBA0.05 mg/L+IAA0.1 mg/L+琼脂5 g/L+蔗糖30 g/L作为生根培养基培养15 d,生根率可达100%;生根苗移栽后全部成活。

The tender leaf blade and stems of Populus deltoides×P.nigra cv.Chile were used as experiment materials for tissue culture research,and the effect of basic culture medium,auxin,cytokinin and other factors on the explants regeneration was studied.The results showed that the optimal medium for differentiation of adventitious roots from the leaf blades was MS ＋ 6-BA 0.25 mg/L ＋ NAA 0.09 mg/L ＋ Agar 5 g/L ＋ Sugar 30 g/L,on which the differentiation rate could reach 99.07%.The suitable medium for inducing the callus with tender stems was 1/2 MS ＋ 6-BA 0.1 mg/L ＋ NAA 0.1 mg/L ＋ IAA 0.1 mg/L ＋ Agar 5 g/L ＋ Sugar 30 g/L,many adventitious buds could differentiate from the callus when transferred to the differentiation medium.The best medium for rooting was 1/2 MS ＋ IBA 0.05 mg /L ＋ IAA 0.1 mg/L ＋ Agar 5 g/L ＋ Sugar 30 g/L,by which the rooting rate was 100% within 15 days,and the rooted seedlings all survived after being transplanted.