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GDF-5诱导成纤维细胞分化为软骨样细胞 被引量：1

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摘要 目的原代分离培养人真皮成纤维细胞(hDFs),应用生长分化因子-5(GDF-5)进行诱导培养,观察细胞的形态,检测软骨细胞相关指标,评价其分化为软骨样细胞的能力。方法取成人除皱术后的皮肤组织,Ⅰ型胶原酶消化法分离培养hDFs,观察细胞形态,MTT法检测细胞增殖活性,免疫细胞化学染色检测Ⅰ型胶原的表达。用100ng/ml GDF-5的成软骨诱导液培养,观察细胞形态变化,检测Ⅱ型胶原蛋白表达及分泌GAG的能力。结果 hDFs呈长梭形,P10以后仍保持较强的增殖潜能,Ⅰ型胶原表达阳性。诱导后的hDFs逐渐成为圆形、多角形,并聚集生长形成软骨样结节;诱导第21 d的细胞Ⅱ型胶原蛋白表达阳性,GAG被甲苯胺蓝染成蓝色。结论 hDFs经100ng/ml的GDF-5诱导培养可分化为软骨样细胞。
作者 王艳秋 高阳 张曼 李德华
机构地区 辽宁医学院人体解剖学教研室 辽宁省盘锦市中心医院
出处 《中国实用医药》 2013年第9期240-242,共3页 China Practical Medicine
关键词 成纤维细胞 生长分化因子-5 软骨样细胞
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]
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参考文献9

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中国实用医药
2013年 第9期

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