摘要
本文建立了发酵乳制品中双歧杆菌种类和数量快速测定方法。以发酵乳中双歧杆菌为靶标,采用PCR-DGGE技术快速识别双歧杆菌种类;采用Real-Tine PCR手段测定双歧杆菌数量。研究结果表明,PCR-DGGE能准确、快速鉴别双歧杆菌种类,检出限为105CFU/g;Real-Tine PCR能准确、快速定量双歧杆菌,检出限为104CFU/g。该方法可用于发酵乳中双歧杆菌的快速识别和定量。
In this article, a rapid identification and numbemtion method for bifidobacterium in fermented milk products was established. Polymerase Chain Reaction-Denaturing Gradient Gel Eleclrophoresis (PCR-DGGE) technique was used to identify Bifidobacterium present in commercial probiotic yoghurts.Real-Time Polymerase Chain Reaction (Real-Time PCR) technique was used to detect Bifidobacteria. The results showed that PCR-DGGE assays enabled identification of the species of bifidobacterium initially present in commercial fermented milk products with a detection threshold of 10^5 cells per gram. Real-Time PCR assays enabled numbering Bifidobacterium with a detection threshold of 10^4 ceils per gram of product. Established PCR-DGGE analysis method was suitable for identification and detection of Bifidobacterium in commercial probiotic yoghurts.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第4期858-862,共5页
Modern Food Science and Technology