发酵乳活性双歧杆菌种类快速识别及定量研究

Isolation of Bifidobacteria from Yoghurts and Assessment of the Bifidobacterial Population by PCR-Denaturing Gradient Gel Electrophoresis and Quantitative Real-Time PCR

本文建立了发酵乳制品中双歧杆菌种类和数量快速测定方法。以发酵乳中双歧杆菌为靶标,采用PCR-DGGE技术快速识别双歧杆菌种类;采用Real-Tine PCR手段测定双歧杆菌数量。研究结果表明,PCR-DGGE能准确、快速鉴别双歧杆菌种类,检出限为105CFU/g;Real-Tine PCR能准确、快速定量双歧杆菌,检出限为104CFU/g。该方法可用于发酵乳中双歧杆菌的快速识别和定量。

In this article, a rapid identification and numbemtion method for bifidobacterium in fermented milk products was established. Polymerase Chain Reaction-Denaturing Gradient Gel Eleclrophoresis （PCR-DGGE） technique was used to identify Bifidobacterium present in commercial probiotic yoghurts.Real-Time Polymerase Chain Reaction （Real-Time PCR） technique was used to detect Bifidobacteria. The results showed that PCR-DGGE assays enabled identification of the species of bifidobacterium initially present in commercial fermented milk products with a detection threshold of 10^5 cells per gram. Real-Time PCR assays enabled numbering Bifidobacterium with a detection threshold of 10^4 ceils per gram of product. Established PCR-DGGE analysis method was suitable for identification and detection of Bifidobacterium in commercial probiotic yoghurts.