桑椹片中多酚及花青素含量的测定

Determination of Polyphenols and Anthocyanins Contents in Mulberry Tablet

采用Folin-Ciocaiteu法测定桑椹片中总多酚含量，通过HPLC法分析其中花青素的主要花青素单体及含量，及运用pH示差法测定其总花青素含量。该桑椹片的总多酚含量63．76mgGAE／g，总花青素含量为6．63mg／g；从HPLC花青素谱图中主要检测到矢车菊素-3-葡萄糖苷、矢车菊素-3-芸香苷这两种单体，其含量分别为2．91mg／g和2．55mg／g，含量之比1．14：1。该方法在2~500μg·mL^-1有很好的线性关系1．00（O-4）．9999，平均回收率102．5--97．8％，RSD为0，93～1．01％。研究结果表明，该方法更为灵敏、准确、重现性好，通过HPLC方法建立，综合了多酚和花青素单体的含量及其比例的测定能够有效地对桑椹片的质量控制。

The Folin-Ciocalteu method was used to determine the total polyphenol content of mulberry tablet. The main monomer and contents of anthocyanins were determined by HPLC. The pH differential method was applied to determine the total anthocyanins content. The total polyphenol content of Lipid-lowering tablets was 63.76 mg GAE/g and the total anthocyanin content was 6.63 mg/g. The monomer of comtlower-3-glucoside and comflower-3-rutin were the main anthocyanin compounds with thire contents being of 2.91 mg/g and 2.55 mg/g, respectively. And the ratio of the two compounds was 1.14：1. A better linear relation was found in the range of 2-500μg·mL^-1 （R2=1.0000-0.9999） and the average recovery rate was in the range of 102.5-97.8% with the RSD of 0.93-1.01%. The method applied in this research was sensitive, accurate and reproducible, which can be effectively used in the quality control of mulberry tablet.