摘要
采用反相高效液相色谱测定烟草中的茄尼醇,采用外标法进行定量,确定了色谱分离的条件,并通过皂化提取实验确定了茄尼醇最佳皂化条件:58℃、0.6 mol/LKOH甲醇溶液皂化35 min。该法具有良好的线性(相关系数为0.9999),检测限为27.4 ng(S/N=3),加标回收率为99%,方法的RSD为0.41%,方法简单、准确。
A simple and rapid method was developed for determination of solanesol in tobacco by RP-HPLC and the saponification process was optimized. The solanesol content was quantitatively determined with the external standard method. The results indicated that the optimum saponification process was as follows： KOH -methanol solution 0.5 mol/L, of saponification temperature 30 ℃, and the saponification time 30 minutes. The method had good linearity with correlation coefficient of 0.9999. The limit of detection based on signal-to-noise ratio of 3 was 27.4 rig. The recovery ofsolanesol in tobacco was 99% with RSD of 0.41%.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第4期894-897,共4页
Modern Food Science and Technology
