摘要
建立了高效液相法同时测定中药材人参中多茵灵、甲基硫菌灵农药残留含量农药残留量方法。色谱条件:DiamonsilC18色谱柱,流动相为甲醇:水,梯度洗脱,检测波长为285nm。结果平均回收率多菌灵为85.73%,RSD为2.07%(11=6);平均回收率甲基硫菌灵为85.83%,RSD为2.39%(n=6)。多菌灵在0.2--1.0μg时,r=0.9990,呈良好的线性关系,甲基硫菌灵在0.2~1.0μg时,r=0.9993,呈良好的线性关系。本方法简便、快速、适合同类中药材中多菌灵、甲基硫茵灵农药残留量测定与安全监控。
A High performance liquid chromatography (HPLC) method was developed in this paper for simultaneous determination of residual carbendazimin and Thiophanate-methyl in Ginseng, a Chinese traditional medicine.The HPLC analysis of the samples were carried out using methanol-water as mobile phase on a Diamonsi C18 column at 285 nm. The average recovery and RSD for Carbendazimin were 85.73% and 2.07% (n=6), respectively. The average recovery and RSD for Thiophanate-methyl were 85.83% and 2.39% (n=6), respectively. The standard curve of carbendazim was linear in the range of 0.2-1.0 μg (R=0.9990)and the standard curve of Thiophanate-methyl was linear in the range of 0.2~1.0 μg (R=0.9993). This method was simple, rapid and reliablel, which is suitable for the determination and safe control of residual carbendazimin and Thiophanate-methyl in Chinese traditional medicines.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第4期907-910,共4页
Modern Food Science and Technology
关键词
人参
多菌灵
甲基硫菌灵
农药残留
高效液相
ginseng
carbendazim
thiophanate-methyl and residualpesticide
HPLC