耐药性木霉T2菌株的筛选、紫外诱变与药剂驯化

UV mutagenesis and screening for fungicide resistant strains of Trichoderma aureoviride T2

为获得对杀菌剂有抗药性的菌株,首先测定了深绿木霉T2菌株对6种常用杀菌剂的抗性,选择出了最敏感的杀菌剂-速克灵。同时通过紫外光照射挑选出突变菌株,并在不同浓度的速可灵(50～800μg/mL)培养基上驯化,最后得到了4株对速克灵抗药性较强的菌株:T2-1,T2-2,T2-5和T2-6。10次转化、菌落生长速度测定、产孢数量的测定和对灰葡萄孢菌的拮抗作用测定结果表明,此4个菌株生物学特性稳定,其中T2-6菌株显著优于其他3株。

To obtain fungicide resistant strains,Trichoderma aureoviride strain T2 was tested against six fungicides and the most effective fungicide（Procymidone） was selected for screening against mutagenic strains derived by UV irradiation.The strains were grown on a range of procymidone concentrations（50-800 μg/mL） on a medicinal culture medium.Four strongly resistant strains（T2-1,T2-2,T2-5,and T2-6） were obtained.After the 10 inoculation,colony growth rate,sporulation and antagonism to Botrytis cinerea were assessed and the biological characteristics of the four strains were found to be constant.The T2-6 strain was significantly better than the other three strains.