20个黑麦草品系的SRAP遗传多样性分析

Genetic diversity of 20 ryegrass accessions by SRAP markers

利用SRAP分子标记技术对20个黑麦草品系的遗传多样性进行了分析。结果发现,53个引物组合共扩增出765条带,其中多态性带共597条,多态性比率为78.95%。平均每对引物扩增出的带数和多态性条带数分别为14.4和11.2条。各黑麦草品系间的遗传相似系数在0.485～0.727,平均为0.627。从UPGMA聚类分析的结果来看,以阈值0.63可将这20个品系分为4类。2个多花黑麦草品系并不聚在一类,而是分散在18个多年生黑麦草品系中。另外,UPGMA聚类结果与这些品系的来源基本一致,这说明SRAP标记适用于黑麦草品系间的遗传多样性研究。

Sequence related amplified polymorphism（SRAP） markers were used to analyze the genetic diversity among 20 ryegrass（Lolium） lines.The results showed that 765 fragments were amplified by 53 primers.Polymorphic bands were 597 which accounted for 78.95% in the total amplified fragments.The number of amplified and polymorphic fragments was 14.43 and 11.26 per primer,respectively.The genetic similarity coefficients ranged from 0.485 to 0.727 among these ryegrass lines and the average was 0.627.Using unweighted pair-group method with arithmetic means（UPGMA） cluster analysis on the SRAP markers,these ryegrass lines could be divided into four groups by the threshold of 0.63.Two Lolium multiflorum lines could not be clustered into a group but spread in 18 Lolium perenne lines.In addition,cluster results were consistent with the resources of these ryegrass lines.All results suggested that SRAP markers were suitable for the research of the genetic diversity on ryegrass.