泽泻指纹图谱研究

Research of chromatographic fingerprint on Alisma orientalis

建立泽泻饮片的高效液相指纹图谱,研究不同购买地泽泻饮片的质量,为科学评价及有效控制其质量提供参考.使用Zorbax Extend-C18(250mm×4.6mm,5μm)色谱柱,以乙腈-水为流动相采用梯度洗脱法进行色谱分离.11批样品得到的色谱指纹图谱有25个共有峰,其中3,5,6,8,10,12号峰分别被鉴定为泽泻醇F,24-乙酰泽泻醇F,23-乙酰泽泻醇E,24-乙酰泽泻醇A,25-去羟基泽泻醇A,11-去氧泽泻醇A.首次使用泽泻中多个三萜类成分作为对照品对指纹图谱色谱峰进行指认,结果重现性好,为控制泽泻饮片的质量提供可靠的分析方法.

To develop a HPLC method of the chromatographi quality control. The HPLC chromatographic profiles Column（250 mm× 4. 6 mm, 5 μm）eluted with the gradient mode. The HPLC chromatographic fingerpr from 11 lots of A. orientalis. Six peaks were identifi alisol E, 24-acetyl alisol A, 25-dehydroxy alisol A an were d mobile c fingerprint of Alisma orientalis for etermined on the Zorbax Extend-C18 phases of water and acetonitrile in int with 25 co ed as alisol F, d 11-deoxyalis mmon peaks was establi 24-acetyl alisol F, 23-a shed cetyl ol A. The HPLC fingerprint method is repeatable, feasible for the quality control of A. orientalis and the preparations.