松树营养物质及次生代谢物质含量与思茅松毛虫危害关系的判别分析

DiscriminantAnalysis for the Relationship between the Damaged Degree of Dendrolimus kikuchii Matsumura and the Contents of Nutrition and Secondary Metabolites in Pine Needles

为了掌握思茅松毛虫[Dendrolimus kikuchii(Matsumura)]的发生危害及其与寄主植物松树之间的关系,了解松树受害后应激反应的变化过程,探讨其与抗性有关的营养指标以及次生代谢物质的关系,以期提出有效的防治技术措施。在福建光泽华桥林场和南平市茂地乡两地分别设置4块样地,轻、中、重度受害及未受害各1块,在2008年4月中旬和8月中旬,采集思茅松毛虫幼虫不同危害程度的松针,测定松针营养物质及次生物质含量,进行判别分析。结果分析表明,松树在思茅松毛虫危害胁迫下,随着受害程度的加重松针内黄酮、单宁、总酚含量呈上升趋势,可溶性糖、多糖、蛋白质含量呈现下降趋势。依据有关指标进行判别分析,能够准确地对松树被害程度进行分级。由此得出结论:松树营养物质以及次生代谢物质与松树危害程度有密切相关,能够反映其变化指标的重要性依次是:总酚、多糖、单宁、黄酮、蛋白质、可溶性糖。判别分析对于多指标、多对象、多组别的分析,较方差分析和多重比较综合性强,能体现多指标的共同作用。

In order to find out the relationship between Dendrolimus kikuchii Matsumura and its host plants,to learn the change process of the pine’s stress response after the plants had been damaged,to explore the nutrients and secondary substances that related to its resistance towards the damage by the pest,and to propose effective measures for prevention and pest-control technology,four sample plots were set up separately in two places including Guangze Forest Farm of Returned Overseas Chinenes and Maodi Town in Nanping City of Fujian Province,including one mild site,one moderate site,one heavily damaged site and one undamaged site,respectively.In mid-April and mid-August of 2008,the pine needles with different damaged-degrees were collected,the contents of the nutrients were examined,and secondary substances was discriminately analyzed.The results showed that when pines were damaged by Dendrolimus kikuchii Matsumura,the contents of tannin,phenol in the pine needles showed an accent tendency as the damaged-degree increased,but soluble sugar polysaccharide,flavanone and protein decreased.The classification of damaged degree could be done accurately based on the discriminant analysis of the indicators.It could be concluded that the indicators tested had close relationship with the damaged degree of pine.The important indicators were phenol,polysaccharide,tannin,flavanone,protein and soluble sugar as orders.The discriminant analysis was more comprehensive than the variance analysis and multiple comparisons for the multi-indicators,multi-objects and multi-groups.The method could give the interaction of multiple indicators comprehensively.