摘要
以紫花苜蓿为材料,研究了不同外植体和不同激素处理对苜蓿愈伤和胚状体分化的影响。结果表明,子叶和下胚轴作外植体,在MS+2,4-D 2.0mg/L+NAA 1.0mg/L+BA 0.5mg/L培养基上愈伤组织诱导率较高,下胚轴的诱导率要高于子叶;下胚轴在MS+BA 3.0mg/L培养基中胚状体分化率最高。
The hypocotyls and cotyledon of alfalfa were used to study the effect of different mediums and hormones. The results indicated that the hypocotyls induction rate was the highest; the best medium for callus induction was MS＋2,4-D 2.0mg/L＋NAA 1.0mg/L ＋BA 0.Stag/L; the best medium for embryoid induction was MS＋ BA 3.0mg/L.
出处
《安徽农学通报》
2013年第7期51-52,共2页
Anhui Agricultural Science Bulletin
关键词
紫花苜蓿
组织培养
快繁技术
Alfalfa
Tissue culture
Rapid propagation technology
