桃果实总蛋白质双向电泳优化体系的建立

Optimization of Two-Dimensional Electrophoresis Conditions for Proteome Analysis of Peach Fruit

以“晖雨露”水蜜桃为试验材料，建立了一种适用于桃果实总蛋白质的双向电泳体系。最终试验确定采用酚抽法提取桃果实蛋白质，采用17cm，pH4-7的线性IPG胶条，800μg蛋白质上样，并用考马斯亮蓝G-250胶体考染，可以得到背景清晰、分辨率高的双向电泳图谱。对影响双向电泳图谱质量的因素进行了讨论分析。

An effective two-dimensional electrophoresis system was developed to determine the total protein from ＂huiyulu＂ peach fruit in this study. The optimum conditions for total protein detection were listed as follows： phenol extraction,800μg protein sample, 17 cm IPG strip with pH 4-7,and finally detecting proteins with modified colloidal coomassie brilliant blue G-250 staining. With the optimum conditions,a high quality 2-DE map with low background was achieved.