复方丹参滴丸降低尿酸和内皮保护作用动物实验研究

Role of compound red sage root dripping pill in reducing uric acid (UA) and protecting endothelium: An animal experimental study

目的探讨复方丹参滴丸对高尿酸血症(hyperuricemia,HUA)动物模型血尿酸(uricacid,UA)的降低作用及对血管内皮的保护作用,为临床降UA治疗提供新途径。方法 38只SPF级雄性昆明小鼠,按体质量随机分为3组:对照组(A)12只(酵母膏+乙胺丁醇+蒸馏水灌胃);别嘌呤醇组(B)13只(酵母膏+乙胺丁醇+别嘌呤醇灌胃);复方丹参滴丸组(C)13只(酵母膏+乙胺丁醇+复方丹参滴丸灌胃)。3周后测定各组血清UA和一氧化氮(NO)水平;分离胸腹主动脉,石蜡包埋、切片后HE染色,并进行免疫组化检测核转录因子-κB(NF-κB)和血管内皮细胞黏附因子-1(VCAM-1)的蛋白表达。结果别嘌呤醇组和丹参滴丸组血清UA水平[(372.71±22.16)μmol/L和(328.65±28.78)μmol/L)]与对照组[(605.67±54.76)μmol/L]相比均有显著下降(P<0.01),血清NO水平[(29.55±1.55)μmol/L和35.11±2.03μmol/L)]与对照组[(20.89±1.70)μmol/L)]相比均升高(P<0.01),复方丹参滴丸组优于别嘌呤醇组(P<0.01)。对照组主动脉内膜NF-κB和VCAM-1可见连续阳性表达,其余两组表达不同程度减弱。各组主动脉内膜形态和血管壁结构均未发生改变。结论复方丹参滴丸可降低HUA小鼠血清UA水平,提升血清NO水平,效果均优于别嘌呤醇;同时可抑制HUA小鼠主动脉内膜NF-κB和VCAM-1的表达。

Objective To provide a new therapy for reducing uric acid（UA） by studying the role of compound red sage root dripping pill in reducing UA and in protecting endothelium in a hyperuricemia animal model.Methods Thirty-eight SPF-class male Kunming mice were randomly divided into control group（group A,n=12）,allopurinol treatment group（group B,n=13）,and compound red sage root dripping pill treatment group（group C,n=13）.Three weeks after the animals in 3 groups were treated with yeast ＋ ethambutol ＋ distilled water gastric lavage,allopurinol ＋ ethambutol ＋ allopurinol gastric lavage,and yeast ＋ ethambutol ＋ compound red sage root dripping pill gastric lavage,respectively,their serum UA and NO levels were measured.Thoracicoabdominal aorta was isolated and cut into sections which were stained with HE.Expressions of NF-κB and VCAM-1 were detected with immunohistochemical staining.Results The serum UA level was significantly lower whereas the serum No level was significantly higher in groups B and C than in group A [（372.71±22.16） μmol/L and（328.65±28.78） μmol/L vs（605.67±54.76） μmol/L,P 0.01;（29.55±1.55） μmol/L and（35.11±2.03） μmol/L vs（20.89±1.70） μmol/L,P 0.01）].The NF-κB and VCAM-1 were strongly expressed in aorta endomembrane of group A but weakly expressed in groups B and C.No significant change occurred in aorta endomembrane morphology and blood vessel wall structure of 3 groups.Conclusion compound red sage root dripping pill can reduce the serum UA level,down-regulate the expression of NF-κB and VCAM-1,and increase the serum NO level in hyperuricemia mice.