269例下肢溃疡病原菌耐药性监测

MONITORING ON ANTIBIOTIC RESISTANCE OF PATHOGENS FROM 269 CASES OF LEG ULCER

目的观察269例下肢溃疡病原菌耐药性,为合理使用抗菌药物提供依据。方法通过细菌分离鉴定和药敏试验方法,对病人溃疡面标本进行了分离培养和药物敏感性测定。结果在269例下肢溃疡的患者创面上共分离出389株致病菌,其中革兰阳性菌占51.4%,革兰阴性菌占32.4%,厌氧菌占15.2%,真菌占1.0%。金黄色葡萄球菌对青霉素G、红霉素及氨苄西林/舒巴坦的耐药率分别为91.3%、70.4%和66.1%;革兰阳性菌仅对万古霉素全部敏感。革兰阴性菌仅对丁胺卡那霉素具有较高的敏感性。结论下肢溃疡患者创面感染以革兰阳性菌为主,所分离出的病原菌对临床常用抗菌药物普遍耐药。

Objective To observe the antibiotic resistance of bacteria isolated from 269 cases of leg ulcers so as to provide the basis for the rational use of antibiotics.Methods The bacteria isolation and identification and drug-sensitive methods were used to analyze samples isolated from leg ulcer.Results A total of 389 strains of pathogens were isolated from 269 leg ulcers.Among the 389 strains,51.4% were Gram-positive bacteria,32.4% were Gram-negative bacteria,15.2% were anaerobes and 1.0% were fungi.Staphylococcus aureus were highly resistant to Penicillin G（91.3%）,Erythromycin（70.4%） and Ampicillin/Sulbactam（66.1%）.Vancomycin was effective against all Gram-positive bacteria,while Amikacin was the only effective agent against the Gram-negative bacteria.Conclusion Gram-positive bacteria are the predominant pathogens isolated from leg ulcers.The isolated pathogens are generally resistant to antibiotics commonly used in clinic.