HPLC法测定腹可安片中东莨菪内酯的含量

Content Determination of Scopoletin in Fukean Tablets by HPLC

目的:建立测定腹可安片中东莨菪内酯含量的方法。方法:采用高效液相色谱法。色谱柱为Kromasil C18柱,流动相为乙腈-0.1%磷酸水溶液(23:77,V/V),检测波长为346nm,流速为1.0ml/min,柱温为25℃,进样量为10μl。结果:东莨菪内酯检测质量浓度在10.25～82.00μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.9995);平均加样回收率为99.6%,RSD=0.6%(n=6)。结论:该方法简便、专属性好、结果准确,可用于腹可安片的质量控制。

OBJECTIVE： To establish the method for the content determination of scopoletin in Fukean tablets. METHODS： HPLC method was adopted. The determination was performed on Kromasil C18 column with mobile phase consisted of acetoni- trile-0.1% phosphoric acid solution （23：77, V/V） at the flow rate of 1.0 ml/min under a detection wavelength of 346 nm. The col- umn temperature was 25℃ and injection volume was 10 μl. RESULTS： The linear range of scopoletin was 10.25-82.00 μg （r= 0.999 5） with an average recovery rate of 99.6% （RSD=0.6%, n=6）. CONCLUSION： The method is simple, specific and accurate, and it can be used for the quality control of Fukean tablets.