霉酚酸酯对糖尿病大鼠肾脏的保护作用及其对MIF、MCP-1表达影响的研究

The effect of mycophenolate mofetil on the renal protection and the expression of MCP1 and MIF in diabetic rats

目的研究霉酚酸酯对糖尿病大鼠的保护作用,并探讨其作用机制,对MCP-1、MIF表达的影响。方法选取雄性Wistar大鼠55只,行右肾切除术后两周,随机分成模型组和正常对照组(A组),将糖尿病模型组大鼠随机分为糖尿病大鼠对照组(B组)和糖尿病大鼠治疗组(C组)。应用酶免疫分析法测定大鼠24h尿白蛋白排泄率,采用HE、PAS染色,行肾脏组织形态学分析,用Real time FQ-PCR检测MCP-1、MIF等细胞因子的mRNA表达情况。结果和B组(13.71±0.62)相比,C组大鼠尿白蛋白排泄率明显下降(4.32±0.30),差异具有统计学意义(P<0.05);C组KW/BW、MGV、FMA比B组有不同程度的下降(P<0.05);C组大鼠肾脏MCP-1、MIF的mRNA表达有所下调(P<0.05),但仍高于A组;MCP-1、MIFmRNA表达水平与UAER成正相关(r=0.703,P<0.05;r=0.657,P<0.05);MCP-1mRNA表达水平与KW/BW、MGV、FMA成正相关(r=0.651,P<0.05;r=0.639,P<0.05;r=0.771,P<0.05);MIFmRNA表达水平与KW/BW、MGV、FMA成正相关(r=0.567,P<0.05;r=0.593,P<0.05;r=0.876,P<0.05)。结论霉酚酸酯对糖尿病大鼠肾脏可起到保护作用,其机制可能与下调MCP1、MIFmRNA的表达有关。

Objective：To assess the renal protective effects and the expression of MCP1mRNA and MIF mRNA of myclate mofetil（MMF） in diabetic model rats.Methods：55 Male Wistar rats were randomized into the following groups：（A） normal control;（B） diabetic control;（C）diabetic treatment group（MMF group）.24h urinary albumin excretion rate（UAER） was detetcted by Enzyme immunoassay;The renal patho-logical changes of renal tissue were observed by HE,PAS stain;Expressions of MCP-1 and TIMP-1mRNA in renal tissue were detected by Real time FQ-PCR.Results：UAER of group C（4.32±0.30）was significantly lower than that of group B（13.71±0.62）（P 0.05）;KW/BW,MGV and FMA of group C were significantly lower than that of group B（P 0.05）;Expressions of MCP-1 and MIFmRNA of group C were significantly lower than group B,but still higher than group A;and which were possotively correlated with 24h UAER,KW/BW,MGV and FMA（P 0.05）.Conclusion：MMF has protective effect to diabetic kidney.The mechanism may be correlated with down-regulating the expression of MCP-1 and MIF.