半夏总生物碱对人乳腺癌细胞增殖的抑制作用

Inhibitory Effect of Total Alkaloids from Pinellia Ternate on the Proliferation of Human Breast Cancer cells

目的探讨半夏总生物碱(total alkaloids from pinellia ternate,TATP)对人乳腺癌细胞株MDA-MB-435S增殖的影响。方法采用四甲基偶氮唑盐(methyl thiazolil tetracolium,MTT)比色法以及集落形成率实验,检测不同浓度的TATP对MDA-MB-435S细胞株的生长抑制作用。应用单细胞凝胶电泳分析检测TATP导致MDA-MB-435S细胞的DNA损伤情况。结果人乳腺癌细胞MDA-MB-435S经TATP处理后,其体外增殖能力受到明显抑制且与药物剂量、作用时间呈正相关,经TATP作用24、48、72h后的半数抑制浓度分别为:98.37μg/ml、52.16μg/ml、33.63μg/ml。单细胞凝胶电泳(single cell gel electrophoresis,SCGE)中,TATP组细胞尾DNA含量、尾长及尾动量与对照组有统计学差异(P<0.01),且呈现浓度依赖性。结论在体外培养条件下,TATP能明显抑制MDA-MB-435S细胞增殖,其机制可能与DNA的损伤作用有关。

Objective To investigate the proliferation inhibition effect of total alkaloids from Pinellia ternate（TATP） on the human breast cancer line MDA-MB-435S.Methods The proliferation inhibition effect of different concentrations of TATP on the human breast cancer cell line MDA-MB-435S was measured by methyl thiazolil tetracolium colorimetic（MTT） method and plate clone formation assay.The DNA damage was detected by the single cell gel electrophoresis（SCGE）.Results The proliferation in MDA-MB-435S cell treated with TATP was decreased significantly compared with the control group and the proliferation inhibition was positively correlated to the TATP concentration and the reaction time.The IC50 of MDA-MB-435S cell incubated 24,48,72 hours with TATP were 98.37 μg/ml,52.16 μg/ml and 33.63 μg/ml respectively.Compared with the control group,the tail DNA content,the tail length and the tail movement of the TATP experimental groups were significantly different（P0.01） and presented a dose-effect relationship.Conclusion TATP could inhibit the proliferation of MDA-MB-435S cells in vitro,which might be correlated to the DNA damage induced by TAPT.