胃窦癌患者幽门螺旋杆菌感染率与MiR-21表达水平的相关性

Relationship between Helicobacter Pylori Infection Rate and Expression of MiR-21 in Patients with Gastric Antral Cancer

目的探讨胃癌患者幽门螺旋杆菌(Helicobacter pylori,Hp)感染率与miR-21表达水平的相关性。方法2011-01/2012-01月求诊广州军区广州总医院消化内科的患者,经电子胃镜检查,活检其病变组织送病理检查,纳入诊断为胃窦癌的患者91例,慢性浅表性胃窦炎患者95例。两组患者均行13 C呼气试验,明确Hp感染情况。胃窦癌患者转外科行手术根治,术中留取病变组织及癌旁组织,应用实时荧光定量聚合酶链反应(real time fluorescence qua-ntitativepolymerase chain reaction,qRT-PCR)进行miR-21表达水平检测。结果①胃癌患者Hp感染率为79.12%,高于慢性浅表性胃窦炎组的66.32%,差异有统计学意义(P=0.001);②胃癌患者的胃癌组织miR-21表达水平较癌旁组织高,差异有统计学意义(P=0.000);③感染Hp的胃癌患者miR-21表达水平较Hp阴性患者高,差异具有统计学意义(P=0.009)。结论Hp感染、miR-21高表达可能共同参与胃癌的发生、发展,二者存在相关性。

Objective To study the relationship between the expression of miR-21 and the infection rate of Helicobacter pylori（Hp） in patients with gastric antral cancer.Methods A total of 91 patients diagnosed as having gastric cancer by electronic gastroscopy and pathological examination and 95 patients with chronic superficial gastritis who visited Guangzhou General Hospital of Guangzhou Command during January 2011 to January 2012 were studied.13C urea breath tests were used to investigate the Hp infection.Quantitative real time fluorescence qua-ntitative polymerase chain reaction（qRT-PCR）was performed to detect the miR-21 expression level of the tissue obtained during operations.Results The infection rate of Hp in gastric cancer group was 79.12%,which was significantly higher than 66.32% in the chronic superficial gastritis group（P=0.001）.The expression of miR-21 in gastric carcinoma tissue was significantly higher than that in the tumor-adjacent normal colorectal tissue（P=0.000）.The expression of miR-21 in gastric carcinoma of Hp（＋） patients was significantly higher than that in the Hp（-） patients（P=0.009）.Conclusion Hp infection and high level of miR-21 may contribute to the occurrence and development of gastric cancer.