大鼠代谢型谷氨酸受体第5亚型基因片段的克隆

CLONING OF THE FRAGMENT OF RAT mGluR_5 GENE

从大鼠的尾壳核组织中提取总 RNA,以 RT-PCR方法扩增出大鼠 m Glu R5 长度约 43 5 bp的 c DNA片段。将这一片段克隆到 PGEM-T载体中进行序列分析 ,结果证实所克隆的 c DNA是编码正确的大鼠 m Glu R5 的一段基因序列。克隆的这段大鼠m Glu R5 特异性基因片段可用于制作探针 ,利用原位杂交技术检测其 m RNA在正常或异常状况下的表达 ;也可制作反意 c DNA或反意 m RNA以研究 m Glu R5 在生理或病理状态下的作用 ;还可进行反意 c DNA或反意 m RNA基因治疗。总而言之 ,克隆的这段基因 ,对研究 m Glu R5 在生理及病理条件下的功能变化 。

Brain tissues of caudate nucleus and putamen were used to extract total RNA. A 435 bp positive cDNA band was obtained by RT PCR method. This fragment was cloned into the PGEM T vector and sequenced. The result shows that the sequence is correct. The cloned fragment may be used to synthesize probes for analysis of mGluR 5 mRNA expression under normal or abnormal conditions. Also, the cloned cDNA may be used to produce antisense cDNA and antisense mRNA for research of mGluR 5 physiological and pathological action as well as gene therapy. In a word, the cloned rat mGluR 5 gene fragment will play an important role in research of function change of mGluR 5 in various conditions,and in basic research and clinical utility of mGluR 5 in some diseases.