电针、NGF对臂丛前索损伤再生过程中脊髓运动神经元GAP-43表达的影响

EFFECT OF ELECTROACUPUNCTURE AND NGF ON THE EXPRESSION OF GAP-43 IN THE SPINAL MOTONEURONS DURING INJURY AND REGENERATION OF THE BRACHIAL PLEXUS

应用免疫组织化学方法 ,观察了豚鼠臂丛前索损伤与再生过程中脊髓运动神经元生长相关蛋白 ( GAP-4 3 )的表达以及电针、NGF对其表达的影响。结果表明 ,未受损神经的神经元 GAP-4 3呈弱阳性表达 ;但 GAP-4 3在神经损伤初期的表达显著增强 ,随着轴突不断生长 ,表达强度又逐渐减弱 ;电针、NGF能够加快其减弱速度 ,特别是电针能够显著加快 GAP-4 3恢复弱阳性表达。由此可以认为 ,神经元 GAP-4 3含量变化不仅能够反映轴突生长锥生长与靶器官的接触 ,还可表达再生轴突与靶器官之间功能联系的完善程度。电针促进其完善的作用优于 NGF。

In this article, the expression of GAP 43 in the spinal motoneurons during injury and regeneration of the brachial plexus and the effects of electroacupuncture(EA) and NGF on it were studied by immunohistochemical methods. The results showed: the expression of GAP 43 was feebly positive in the uninjured neurons. While it was significantly accentuated in the primary stage of injury, and diminished gradually with the regeneration of neurons. Furthermore, this diminishing rate could be accelerated by EA or NGF, especially by the former. In conclusion, the changes of level of GAP 43 in the neurons could reflect not only the contacts of neuroaxon growth cones with their target organs, but also the consummate degree of functional links between the regenerating neuroaxons and their target organs; and the promotion of the formation of functional links between the regenerating neuroaxons and their target organs by EA, which was superior to that of NGF, might be related to the direct stimulation of EA to the target muscle.