乳腺癌细胞免疫逃逸能力的研究

The research of the differences of invasiveness and immune evasion between breast cancer cell lines

目的观察乳腺癌细胞株MCF-7和MDA-MB-231侵袭能力和免疫逃逸能力的差异。方法以Transwell小室进行人工基底膜侵袭实验检测1. 0 × 105个/孔MCF-7细胞和MDA-MB-231细胞侵袭能力的差异;不同浓度混合淋巴细胞与5.0 × 103个/孔乳腺癌细胞株共培养检测MCF-7细胞和MDA-MB-231细胞免疫逃逸能力的差异。结果MDA-MB-231细胞侵袭细胞数量高于MCF-7细胞侵袭细胞数量（86.64 ±7.13比12.27 ±2.53）,差异有统计学意义（P〈0.05）;与2.5 × 104个/孔、5.O× 104个/孔、1. 0 × 105个/孔、2. 0 × 105个/孔混合淋巴细胞共垮养后,MDA-MB-231细胞存活率髙于 MCF-7 细胞存活率[（1.31 ±0.04）% 比（0. 97 ±0. 01）% ；（1.31 ±0.03）% 比（0.80 ±0.01）% ；（1.43 ±0. 00）% 比（0.60 ±0. 01）% ；（1.57 ±0.09）% 比（0. 59 ±0. 01）% ]，差异有统计学意义（P 〈0. 05）。结论MDA-MB-231细胞株较MCF-7细胞株有更强的侵袭能力和免疫逃逸能力。

Objective To study the differences of invasiveness and immune evasion between MCF-7and MDA-MB-231 in vitro. Methods Transwell was used to analyze the difference of invasiveness be-tween 100 thousands per hole MDA-MB-231 and MCF-7 ; Co-culturing with 5 thousands per hole breastcells mixed by various concentrations of lymphocytes was used to observe the difference of immune evasionbetween MDA-MB-231 and MCF-7. Results MDA-MB-231 invaded more cells than MCF-7 （86.64 ±7. 13 vs 12. 27 ±2. 53, P 〈 0. 05） ; The survival rate of MDA-MB-231 was higher than that of MCF-7 co-culturing with 25 thousands per hole, 50 thousands per hole, 100 thousands per hole, 200 thousands perhole lymphocytes [（1.31 ±0.04）% vs （0.97 ± 0. 01 ） % , P 〈 0. 05; （1.31 ±0,03）% vs （0.80 ±0.01）%,P〈0. 05; （1.43 ± 0.00）% vs （0.60 ±0.01）%,p 〈0.05; （1.57 ±0,09）% vs （0. 59 ±0. 01 ）% , P 〈0. 05] . Conclusion Our results suggest that MDA-MB-231 cells display stronger invasive-ness and immune evasion.