白细胞介素-17在糖尿病大鼠肾脏表达及其与α-平滑肌肌动蛋白的关系

Association of interleukin-17 on the expression in the renal of rats with diabete and a smooth muscle actin

目的观察白细胞介素，17（IL-17）在糖尿病大鼠肾组织的表达，探讨其参与。肾小管-间质病变的机制。方法55只雄性sD大鼠，随机选择35只以50mg／kg链脲佐菌素（STZ）制备糖尿病大鼠模型，剩余20只为正常对照组。于2、4周末采用酶联免疫吸附试验（ELISA）方法检测血、尿中IL-17。4周末采用免疫组织化学染色及Westemblot检测。肾组织中IL-17、α-平滑肌肌动蛋白（仅．SMA）及核因子-κBp65（NF．KBp65）。结果糖尿病组大鼠2周末血、尿中IL-17水平分别为（11．51±5．62）、（79．21±14．53）ng／L，与对照组比较差异有统计学意义[（8．87±3．35）、（6．75±1．13）ng／L，P〈0．01]，4周末升高更明显；IL-17在正常对照组肾组织无表达，仅在糖尿病大鼠肾小管和肾间质中高表达。糖尿病大鼠。肾组织中IL-17表达与尿中IL-17（r=0．715，P〈0．05）及肾组织中d．SMA（r=0．708，P〈0．05）、NF—KBp65（r=0．638，P〈0．01）表达均呈正相关。结论IL-17参与糖尿病肾病早期肾小管-间质病变的可能机制为促进仪-SMA表达增加。

Objective To investigate the association between the expression of interleukin-17 （IL-17） in renal tubuleinterstitium and diabetic nephropathy. Methods 55 rats were randomly divided in- to three groups： control group, diabetic rat group. Diabetic rat group was injected 50 mg/kg streptozoticin （STZ）. In the 2th, 4th week, serum and urine IL-17 were measured with enzyme-linked immunosorbent as- say （ELISA）. The expression of IL-17, α-smooth muscle actin （cL-SMA） and nuclear factor-KB p65 （NF-KB p65） in the kidney were assessed by immunohistochemisty staining and Western blotting. Results Serum and urine IL-17 in diabetic rats were [ （ 11.51 ±5.62）, （79. 21 ± 14. 53） ng/L], which were sig- nificantly upreguated as compared with those in control group rats （ P 〈 0.01 ）, which were [ （ 8. 87 ± 3.35）, （6. 75 ± 1.13） ng/L]. The expression of IL-17 was only observed in renal tubuleinterstitium of diabetic rats, the expression of IL-17 in renal tubuleinterstitium of diabetic rats was positively correlated with urine IL-17 （ r = 0. 715, P 〈 0.05 ）, and the expression of ct-SMA （ r = 0. 708, P 〈 0. 05 ）, NF-KB p65 （ r = 0. 638, P 〈 0. 01 ） in renal. Conclusion The effect of IL-17 on pathogenesis and progression of diabetic nephropathy in diabetic rat might be up-regulating the expression of α-SMA.