微小RNA-26b对前列腺癌细胞迁移和侵袭行为的影响

MiR-26b inhibits the prostate cancer cell＇s migration and invasion

目的观察上调微小RNA（miRNA，miR）-26b表达对前列腺癌细胞生物学行为的影响。方法实时荧光定量聚合酶链反应（FQ—PCR）检测miR-26b在5种前列腺癌细胞株和良性前列腺组织中的表达。将miR-26b模拟物和阴性对照miRNA分别转染至miR-26b表达最低的LNCaP细胞。噻唑蓝（MTT）法检测miR-26b表达对LNCaP细胞的增殖能力，流式细胞仪检测其细胞凋亡率，细胞划痕实验和Transwell小室侵袭实验分别检测其细胞迁移和侵袭能力。结果与良性前列腺组织比较，miR-26b在5种前列腺癌细胞株中均呈低表达，以LNCaP细胞株（0．214-t-O．019）最为明显。同阴性对照组比较，转染miR-26b的LNCaP细胞miR-26b表达（65．597±11．426）增强310倍，其迁移细胞数[（29±3）个]和细胞穿膜数[（30±2）个]均较对照组的（158±16）个和（147±15）个显著减少，差异有统计学意义（P〈0．05），而其细胞增殖能力、细胞凋亡率和细胞周期则变化不明显。结论miR-26b在前列腺癌细胞株低表达，上调前列腺癌细胞miR-26b的表达能够抑制前列腺癌细胞的迁移和侵袭能力。

Objective To investigate the effects of the up-regulated expression of microRNA （miRNA, miR）-26b on the biological properties of prostate cancer cells. Methods The expression of miR-26b in PC23, PC-3M, DU-145, LNCaP, 22RV1 cell lines and benign prostate tissue were detected by Real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）. miR-26b mimics and control miRNA were transfected into LNCaP cells by Lipofectamine 2000. The cellular growth activity was assayed by methyl thiazol tetrazolium （MTlr） assay, the apoptosis and cell cycles was tested by flow cytometry. Wound healing assay and Transwell assay were applied to measure cell migration and invasion. Results The expression of miR-26b in prostate cancer cell lines was lower than that in benign prostate tissue. Com- pared to the control, the expression of miR-26b was increased by 310 folds in LNCaP transfected with miR- 26b, which numbers of migrated and penetrated reduce significantly from （ 158 ± 16） and （ 147± 15 ） to （ 29± 3 ） and （30 ± 2） resPectively （ P 〈 0. 05 ）, whereas, cellular growth activity, apoptosis and cell cy- cles were not changed significantly. Conclusion The expression of miR-26b is down-regulation in prostate cancer cell lines. Up-regulation of miR-26b inhibits LNCaP cell＇ s migration and invasion.