酒精性与正常股骨头骨组织中微小RNA基因的多态性分析

MicroRNA genes diversity analysis between bone tissue of alcohol avascular necrosis femoral head and normal femoral head

目的观察微小RNA（miRNA）在酒精缺血坏死性与正常股骨头骨组织的表达变化，探讨miRNA基因在两者中的变化关系。方法分别对5例酒精性股骨头与5例正常股骨头骨组织进行miRNA基因芯片检测，可见有上调基因has．miR-29a．st和下调基因has．miR-486-5p．st，分别对上调和下调基因行逆转录-聚合酶链反应（1iT—PCR）。结果miRNA基因芯片共检测酒精缺血坏死性与正常股骨头骨组织400条基因，对明显上调has—miR-29a—st和下调has．miR-486-5p．st基因片段行RT．PCR检测，正常股骨头和酒精坏死性股骨头上调基因的2-△△ct平均值为1．06±0．09和1．93±1．12，下调基因的2-△△ct平均值为1．88±25．37和1．70±0．98（变化〉2倍且P〈0．05）。结论酒精缺血坏死性与正常股骨头骨组织存在明显miRNA变化，其可能在股骨头坏死发生过程中可能起着重要的调控作用。

Objective To observe expression variation of microRNA （miRNA） between bone tis- sue of alcohol avascular necrosis femoral head and normal femoral head and discuss miRNA change relation between them. Methods It is tested for miRNA for five normal femoral head tissue and alcohol avascular necrosis femoral head tissue, it is thus clear that there are up regulation has-miR-29a-st and downregulation genes has-miR-486-Sp-st, they are went on reverse transcriptase-polymerase chain reaction （RT-PCR）. Results Four hundred genes were analysed between between bone tissue of alcohol avascular necrosis fem- oral head and normal femoral head with miRNA gene chip. clear clown regulation has-miR-486-Sp-st and up regulation has-miR-29a-st were went on PCR, the average value of has-miR-29a-st 2-△△ct is 1.06 ± 0.09 and 1.93 ± 1.12 for bone tissue of alcohol avascular necrosis femoral head and normal femoral head; the average value of has-miR-486-Sp-st2-△△ct is 1.88 ± 25.37 and 1.70 ± 0. 98 （ variation 〉 2 times moreo- ver P 〈 0. 05）. Conclusion There is obvious up regulation and down regulation miRNA genes between bone tissue of normal femoral head and alcohol avascular necrosis femoral head. which is possible to take on main regulation action to .speeding up necrosis femora[ head.