一种新型抗菌强化磷酸钙骨水泥的研究

A new antibacterial and strength-reinforced calcium phosphate cements

目的探讨新型强化型抗菌磷酸钙骨水泥（CPC）的机械及抗菌性能。方法使用经过季铵盐基团改性的壳聚糖丝强化CPC骨水泥，通过涂板技术及激光共聚焦显微镜观察其抗菌效果；通过噻唑蓝（M盯）比色法及4’，6-二脒基-2-苯基吲哚（OAeI）染色观察其生物相容性；通过三点弯曲试验检测其力学性能。结果通过激光共聚焦可见新材料具有较好的抗菌效果，其细菌菌落比例为（0．10±0．09）％；三点弯曲试验表明经过壳聚糖丝改进后的机械性能[（17．3±4．5）MPa与（16．9±3．9）MPa]较之普通骨水泥有明显改善[（5．3±1．4）MPa]；并且这样的改进对材料的生物相容性没有影响，其毒性试验显示新材料相容性比值为（97．5±3．3）％，细胞增殖实验3天吸光度值分别为0．237±0．025、0．503±0．015、0．726±0．03：2。结论新型的季铵盐壳聚糖强化型骨水泥无细胞毒性，具有良好的机械性能及抗菌效果。

Objective To evaluation the mechanical behavior and the ability of antibacterial of the new calcium phosphate bone cement （ CPC ）. Methods The CPC was reinforced by chitosan fiber with hydroxypropyhrimethyl ammonium chloride chitosan （HACC）. The spread plate analysis and confocal laser scanning microscopy were used for bacterial adherence assay. The strength of the samples was investigated by a standard three-point bending test. Cell toxicity and proliferation of cells were also invested. Results The new bone cement showed a good antibacterial activity which had the least amount of surviving bacterial as （0. 10 ± 0. 09 ） %. The results of three-point bending test also showed the incorporation of ehitosan fiber [ （ 17.3 ± 4. 5 ） MPa and （ 16.9 ± 3.9 ） MPa ] significantly increased the mechanical strength of CPC [ （5.3 ± 1.4） MPa ]. And the reinforcement has no harmful to the biocompatibility of CPC. Extracts of new bone cement showed non-cytotoxic （97.5 ±-3.3 ）%. The methyl thiazol tetrazolium （MTT） activity of cells is 0. 237 ± 0. 025, 0. 503 _± 0. 015, 0. 726 ± 0. 032 during 3 days cultured. Conclusion CPC com- bined with chitosan fiber with HACC can meet the needs of strength, biocompatibility and antibacterial ac- tivity.