显微镜下行小切口-单椎板咬除建立大鼠钳夹型脊髓损伤模型

Establishment of the clip compressive spinal cord injury model through small incision and mono- laminectomy under microscope in rats

目的建立显微镜下小切口一单椎板切除后钳夹型脊髓损伤（SCI）模型。方法将30只成年sD大鼠随机分为实验组（n=18）和对照组（n=12），两组均在显微镜下行小切口一胸11椎板咬除，实验组用动脉瘤夹夹闭脊髓2min，对照组不夹闭脊髓。术后进行组织学染色、磁共振（MRI）扫描及BBB评分。结果实验组大鼠术后出现不同程度尿储留和肠功能下降，组织学染色见脊髓结构不规则、片状出血、神经元坏死及髓鞘空泡化。MRI提示出血和水肿并存；对照组未见明显异常；对照组术后BBB评分均为21分，实验组术后1d评分均为0分，1周后为（4．58±0．67）分，2周后恢复至（13．00±1．04）分，各时间点均低于对照组（P〈0．01）。结论显微镜下行小切口一单椎板切除术建立钳夹型脊髓损伤模型具有良好的稳定性和可重复性。

Objective The establish the clip compressive spinal cord injury model through small incision and mono-laminectomy under microscope. Method,s Thirty Sprague-Dawley （SD） rats were ran- domly divided into the injury group （n = 18） and the control group （n = 12）. Small incision and mono- laminectomy at T11 were performed to the injury group, followed by 2-min compressive injury induced by the aneurysm clip. The same procedure was done to the control group except spinal cord injury. Histologi- cal staining was performed one day post-operation, as well as MRI scanning and BBB Locomotor Rating Scale. Results Urinary retention and declination of intestinal function could be witnessed in the injury group, along with hemorrhage, edema, necrosis of neurons and myelin vacuolization histologically. MRI showed mixed signals intramedullarily. None of these were witnessed in the control group. BBB scores in the control group remained at 21 throughout the whole experiment, while those in the injury group possessed 0 immediately after the operation, 4. 58 ±0. 67 and 13.00 ± 1.04 at 1st and 2nd week post-operation re- spectively （ P 〈 0. 01 ）. Conchmion Meticulous operation, small non-related injury and disturbance to the spinal cord are the merits of small incision and mono-laminectomy under microscope.