响应面分析法优化紫芝多糖的脱色工艺

Optimization of Decolorization Technology of Ganoderma sinense Polysaccharides by Response Surface Methodology

根据Box-Behnken实验设计原理,采用3因素3水平的响应面分析法优化紫芝多糖树脂脱色工艺条件,依据回归分析确定各工艺条件的影响因子,以脱色率为响应值作响应面和等高线。结果表明曲面回归模型能较好地预测各因素与响应值之间的关系,优化得到紫芝多糖脱色最佳工艺条件为:脱色时间2.5 h,树脂用量0.75 g/10 ml,脱色温度28℃。在上述条件下,紫芝多糖实际脱色率为87.8%。

Based on the Box-Behnken design principles, the method of response surface methodology with 3 factors and 3 levels was adopted on optimizing the resin decolorization technology of Ganoderma sinense polysaccharides. The factors influencing the technological parameters were determined by means of regression analysis. Response surface and contour were finally graphed with the decolorization rate as the response value. The optimal decolorization conditions were as follows： decolorization time of 2.5 h, 0.75 g/10 ml resin and temprature of 28 ~C. Under such conditions, the actual decolorization rate of Ganoderma sinense polysaccharides was 87.8 %.