Cu^(2+)络合物紫外法测定盐酸氨基葡萄糖含量

Determination of Glucosamine Hydrochloride by UV Spectrophotometry of Cu^(2+) Complex

建立了紫外分光光度法测定盐酸氨基葡萄糖(1)。碱性条件下,1与Cu2+可形成较稳定的络合物而显色,该络合物在紫外区有特定吸收,检测波长为237 nm,1在0.03～0.055 mg/ml范围内线性关系良好。平均回收率为100.1%,RSD为0.57%。

An UV method was established for the determination of glucosamine hydrochloride. In alkaline solution, glucosamine hydrochloride reacted with Cu2＋ to form a stable complex which had a specific absorption wavelength in ultraviolet region. The absorption spectrum of this complex showed a well-defined absorbance maximum at 237 nm. The calibration curve was linear in the range of 0.03 - 0.055 mg/ml. The average recovery was 100.1%, with RSD of 0.57%.