传染性法氏囊病病毒弱毒株对Vero细胞的适应性研究

Proliferation of Infectious Bursal Disease Virus on Vero Cell

传染性法氏囊病病毒疫苗的传统生产工艺是利用鸡胚成纤维细胞增殖 IBDV,但易被外源病原污染 ,且生产成本很高 .借鉴国内外流行性乙型脑炎疫苗生产的经验 ,使用 Vero细胞增殖 IBDV弱毒株 ,从病毒高峰出现时间、滴度峰值的高低、对细胞代谢的影响等方面研究了 IBDV对 Vero细胞的适应性、敏感性以及适应后的种毒的长期保存过程中发现病毒滴度有下降趋势 ,试用了不同试剂对病毒进行保护 .

The adaptation of infectious bursal disease virus (IBDV) B 836 F 4 to Vero cells was studied.Results were as follows:Vero cell was suitable to proliferate IBDV;IBDV titer of the medium reached a high level. Three different additives were tested to protect IBDV during cryopreservation .