米糠多糖的提取纯化及其成分结构和活性分析

Studies of Extraction and Purification Technique of RBS and Anti-tumor Effect Related to the Poly-saccharide

研究米糠多糖 ( rice bran polysaccharide,RBS)的提取纯化方法 ,并对其进行组成成分、化学结构和物理性质分析以及活性测定 .热水抽提法从稻糠中制取粗制 RBS多糖 .层析分离纯化得到精制多糖 .对精制多糖进行元素分析、糖和蛋白含量测定、红外特征吸收光谱测定和碳谱核磁共振测定 ,以及对 Balb/c小鼠同源 Meth- A纤维肉瘤抑瘤活性测定等 .测定结果显示 RBS多糖为一种以 α- 1 ,4和 α- 1 ,6糖苷键为主要化学结构的葡聚糖 ,并对 Meth- A纤维肉瘤有抑制作用 .

Extraction methods and the physico chemical properties and anti tumor effect of RBS were studied. Polysaccharide RBS was prepared from rice bran with hot water extraction. Pure RBS was obtained as a fraction on Sepharose Cl6B. Physico chemical properties of RBS was studied by infrared spectral analysis, 13 C NMR etc.RBS was given to Meth A tumor bearing Balb/c mice to test its anti tumor activity. Physico chemical properties of RBS were as follows: It was a kind of α glucan having α 1,4 and α 1,6 gluco sidic bond as a main frame. It was a white powder soluble in water. Inhibitive effect against Meth A solid tumor was observed by oral administration of RBS.