水稻花药特异启动子Osg6B的序列及功能分析

Sequence and functional analyses of the tapetum-specific promoter Osg6B from rice (Oryza sativa L.)

对水稻花药特异表达基因 Osg6B启动子简称 Osg6B的全序列进行了测定 ,结果表明 ,Osg6B含有 1 .73kb个核苷酸 ,与文献报道的该启动子相差仅 8个核苷酸 ,两者核苷酸同源性为 99.5%。将 Osg6B同 GUS基因编码区相连 ,将构建成的融合基因用基因枪轰击烟草的花药和幼叶 ,荧光分析结果为含 Osg6B的 GUS融合基因在大于 2 mm烟草花药中的表达量比对照和幼叶均高出 8倍 ,在小于或等于 2 mm的花药中 ,则高达到 1 2倍 ,表明 Osg6B启动子具有启动外源基因在植物花药中特异表达的功能。

The tapetum specific promoter ( Osg 6B) from rice was sequenced artificially.The results indicated that the eight nucleotides in the cloned 1.73 kb Osg 6B was different from that the reported.They shared a 99.5% sequence homology.The cloned promoter was fused with the GUS cassette.The resultant construct was introduced into the anther and leaf of tobacco by particle bombardment.The GUS activity in more than two mm anthers was eight times that from leaves and its negative control,and in equal to or less than two mm anthers it was twelve times.These results showed that the cloned Osg 6B promoter performed its function promoting alien gene specitic expressions in plant anthers.