猪瘟病毒弱毒株感染对体外培养细胞增殖的促进作用

EFFECTS OF CLASSICAL SWINE FEVER VIRUS LAPINIZED STRAIN ON ITS HOST CELLS

猪瘟病毒（ＣＳＦＶ）能在多种体外培养细胞中增殖，却不使细胞产生病变（ＣＰＥ）。使用原代细胞增殖ＣＳＦＶ弱毒疫苗。结果显示：病毒的增殖能够增加原代牛睾丸细胞的传代次数和维持时间。因此，ＣＳＦＶ疫苗生产上能够在接毒后多次收获病毒。此外，ＣＳＦＶ弱毒株还能够刺激体外培养的兔巨噬细胞增殖，使形成致密的单细胞层。使用传代细胞 ＰＫ１５增殖病毒，经流式细胞术检测发现 ＣＳＦＶ弱毒的增殖不仅不改变ＰＫ１５细胞的增殖周期，并且能够影响Ｇ４１８对ＰＫ１５细胞增殖的抑制作用，促进其宿主细胞的增殖。ＣＳＦＶ对体外培养细胞的增殖有促进作用，然而感染猪对猪机体的损伤却很严重。实验结果显示ＣＳＦＶ与其宿主细胞间相互作用的复杂性。

Classical Swine Fever Virus(CSFV) can multiplicated in many kinds of culture cells, and its multiplication does not cause cytopathic effects(CPE) on their host cells. Cells can multiply with CSFV inside, and the exitence of the lapinized strains of CSFV increase the passage number of cultured primary bovine testicular cells. In the vaccine production of CSFV, live virus vaccine can be ingathered many times once cells are inoculated. Besides, the lipinized Chinese strain of CSFV mad stimulates the proliferation of the macrophage cells. Flow cytometry showed that the cell cycle of CSFV-infected PK15 cells is not affected by the multiplication of CSFV. Furthermore, the multiplicaton of CSFV can improve cell growth rate and reduce the disturbance of G418 to the normal cell cycle. Considering the severe damage to pigs caused by CSFV, it deduced the complicated interachon between CSFV and its host cells.