高产油脂酵母菌选育及摇瓶发酵条件的研究

SCREEING OF THE HIGH LIPID PRODUCTION STRAINS AND STUDIES ON ITS FLASK CULTURE CONDITIONS

经紫外线和ＥＭＳ复合诱变选育出一株高产油脂的优良酵母菌株，命名为Ｌｉｐｏｍｙｃｅｓ．ｓｔａｒｋｅｙｉ ＨＬ。通过摇瓶培养，对各项与菌体产油脂相关的因素作了单因子实验，确定了摇瓶发酵培养的最佳产油脂条件：碳源，废糖液１６５．７ｍｌ／Ｌ；氮源，硫酸铵１．０８ｇ／Ｌ；Ｃ／Ｎ：６１：１；培养温度为２８℃；接种量１０％；发酵时间９６ｈ； ｐＨ５．０。最后可得油脂产量 ５．９ｇ／Ｌ；菌体生物量 １１．０ｇ／Ｌ；油脂含量 ５３．６％。对菌体内油脂组成进行了气相色谱与质谱分析，结果如下：软脂酸３３．２％，棕榈油酸３．４％，硬脂酸６．０％，油酸５０．８％，亚油酸３．５５％，亚麻酸１．５３％，其它１．５２％。

A mutant of Lipomyces starkeyi was obtained through multiple mutagenesis (Ultraviolet and EMS) and named as L. starkeyi HL, which has the capability of accumulating substantial amount of lipid under N4indted culture conditions. Then seveal factors influencing the lipid accumulation were investigated and the optimum conditions of producing lipid were obtained: concentrations of waste sugar and the nitrogen source (NH4),SO4 were 165.7ml/L and 1.08g/L; C/N 61:1; initial pH5.0; inoculation volume 10%; temperature 28t; culture time 96h. This research analyzed the lipid from the mutant L starkeyi HL with MS/GC, it's content fatty acid were palmitic acid 33.2%, palmitoleic acid 3.4, steric acid 6.0%, oleic acid 50.8%, linoleic acid 3.55%, lindenic acid 1.53%, others 1.52%.