摘要
经紫外线和EMS复合诱变选育出一株高产油脂的优良酵母菌株,命名为Lipomyces.starkeyi HL。通过摇瓶培养,对各项与菌体产油脂相关的因素作了单因子实验,确定了摇瓶发酵培养的最佳产油脂条件:碳源,废糖液165.7ml/L;氮源,硫酸铵1.08g/L;C/N:61:1;培养温度为28℃;接种量10%;发酵时间96h; pH5.0。最后可得油脂产量 5.9g/L;菌体生物量 11.0g/L;油脂含量 53.6%。对菌体内油脂组成进行了气相色谱与质谱分析,结果如下:软脂酸33.2%,棕榈油酸3.4%,硬脂酸6.0%,油酸50.8%,亚油酸3.55%,亚麻酸1.53%,其它1.52%。
A mutant of Lipomyces starkeyi was obtained through multiple mutagenesis (Ultraviolet and EMS) and named as L. starkeyi HL, which has the capability of accumulating substantial amount of lipid under N4indted culture conditions. Then seveal factors influencing the lipid accumulation were investigated and the optimum conditions of producing lipid were obtained: concentrations of waste sugar and the nitrogen source (NH4),SO4 were 165.7ml/L and 1.08g/L; C/N 61:1; initial pH5.0; inoculation volume 10%; temperature 28t; culture time 96h. This research analyzed the lipid from the mutant L starkeyi HL with MS/GC, it's content fatty acid were palmitic acid 33.2%, palmitoleic acid 3.4, steric acid 6.0%, oleic acid 50.8%, linoleic acid 3.55%, lindenic acid 1.53%, others 1.52%.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第2期93-97,共5页
Microbiology China
关键词
废糖液
发酵条件
油脂含量
油脂酵母
微生物油脂
L.starkeyi HL, Waste sugar Fermentation conditions, Lipid component and contents