摘要
研究了摇瓶培养条件下少动鞘脂单胞菌引的胞外多糖的发酵工艺。Ｓ１菌的发酵产胶可采用二步发酵法：第一阶段，培养基成分为：蔗糖１０ｇ，ＮＨ_４ＮＯ_３ ０．５ｇ，ＫＨ_２ＯＰ_４ ０．５ｇ，ＭｎＳＯ_４ ０．３ｇ，ＭｇＳＯ_４ ０．１ｇ，吐温８０ ０．０２ｇ溶于蒸馏水并定容至１０００ｍＬ，ｐＨ７．２±０．１，温度３３℃～３５℃，高溶氧。第二阶段，发酵 １０～１２ｈ后，补加蔗糖４０ｇ／Ｌ，温度 ２４℃～２５℃，ｐＨ６．５±０，１，高溶氧（激烈振荡）。Ｓ１菌胞外多糖的产量最高可达８．９８ｇ／Ｌ，发酵液粘度最高可达４５０００ｍＰａ·ｓ。
Nutritional requirements and optimal condition for exopolysaccharide (EPS) produchon by Sphingomonas paucimobilis S1 were studied. A two-stage fermentation process was conducted as follows: in the earlier stage which lasted for 10-12h, the initial medium contained the following: sucrose 10g, NH_4NO_3,0.5g, KH_2PO_4 0.5g, MnSO_4 0.3g, MgSO_4 0.1g, Tween 80 0.02g, which is dissolved in distilled water to 1000ml, the pH and temperature were 7.2 ± 0.1 and 33-35℃ respectively; in the successive stage, 40g/L of sucrose was added into the broth, and the pH and tempethere were 6.5±0.1 and 24-25℃ respectively.In both stages, highly resolved O_2 (vigorous agitation) was required. Maximum yield of the EPS (8.98g/L) and maximum viscosity of the broth (45000mPa· s) were observed under optimal condition.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第2期97-100,共4页
Microbiology China
基金
广东省自然科学基金会
华南生物科学与技术研究中心资助!No.960257
