抗干扰微生物检测技术研究

RESEARCHES ON ANTI-DISTURBANCE MICROBIAL MEASUREMENT TECHNIQUES

在检测细菌、真菌和大肠菌群时，抗防腐剂型微生物培养基可消除样品中浓度为 ２．０ｇ／Ｌ（Ｋｇ）的山梨酸、山梨酸钾、苯甲酸及苯甲酸钠的干扰；抗消毒剂型微生物培养基可分别消除１５０ｍｇ／ Ｌ二氧化氯、４００ｍｐ／ Ｌ过氧乙酸、７００ｍｇ／ Ｌ次氯酸钠及１８０ｍｐ／ Ｌ过氧化氢的干扰；抗臭氧型微生物培养基可以消除１０．０ｍｇ／Ｌ的臭氧和余氯的干扰。大样倾注平板法可以取５ｍＬ的样品，适用所有样品的检测；液体大样法可以取１００ｍＬ的样品，适用于所有样品的检测及增菌，特别适用于无色液体样品；最小近似数法可以检出次大样中的细菌及真菌总数和大肠菌群数量，可以用于所有样品的检测，尤其适于有色样品的检测。

In the bacterial, fungous and conform bacterial measurement,the anti-preservative media can eliminate the disturbances of the preservatives 2.0g/L(Kg) sorbic acid, potassium sorbate,benzoic acid and sodium benzoate in the samples, the anti-disinfectant media can eliminate respectively the disturbances of the disinfectants 150mg/L chloride dioxide, 400mg/L peroxyacetic acid. 700mg/L sodium hypochlorite and 180mp/L hydrogen peroxide, and the anti-ozone microbial media can eliminate the disturbances of 10.0mp / L ozone and free chlorine. The big sample pouring plate method can take 5mL sample, suitable for the microbial detection of all kinds of samples. The liquid big sample method can take 100mL sample, suitable for the microbial detection and increasing microbial number of all kinds of samples, especially suitable for the no color liquid samples. The The method can measure the total number of the bacteria, fungi and conform bacteria in the second big samples by applying fifteen tube method or nine tube method. It is suitable for the microbial measurement of all kinds of samples, especially suitable for the microbial measlesment of the samples with color.