不同品系SPF鸡胚细胞对S191株麻疹病毒的适应性和敏感性比较

Adaptivity and Sensitivity of S191 Strains of Measles Virus in Various SPF Chick Embryo Cells

目的 了解我国现有SPF鸡蛋对我国S191株麻疹病毒的可利用性。方法 比较S191株麻疹病毒 ,在我国 3个SPF种蛋供应厂商的 5个品系、6种SPF鸡蛋成纤维细胞上的细胞生长、病变特征和病毒效价方面的差异。结果 LOHMAN F、LOHMAN M、SPAFAS品系SPF鸡蛋的细胞生长发育形态和细胞病变较好 ,其中以SPAFAS为最佳 ;病毒效价 ,SPAFAS和LOHMAN M系在大于 5 .0 0LogCCID50 /ml的高滴度区间的样本数分别达 70 %和 71.96 % ,其中LOHMAN M系的样本都在 4.38LogCCID50 /ml以上的较高水平。结论 我国SPF鸡蛋的SPAFAS和LOHMAN

Objective To explore if the current SPF eggs in China is suitable for the culture of S191 strain of measles virus.Methods The growth and virus titers of S191 strains cultured in 5 lines of SPF chick embryo fibroblast(CEF),as well as the pathological changes of the cells,were compared.Results S191 strain grew well in LOHMAN F,LOHMAN M and SPAFAS cells,and all the cells showed normal pathological changes.Of the 3 lines,SPAFAS is the optimum.The titers of 70% and 71.96% of S191 strains cultured in SPAFAS and LOHMAN M cells were above 5.00LogCCID 50 /ml respectively.However,all the titers of S191 strains cultured in LOHMAN M cells were above 4.38LogCCID 50 /ml.Conclusion Both SPAFAS and LOHMAN M cells are suitable for the culture of S191 strain and production of current measles vaccine.