抑制消减杂交技术在肿瘤转移调控研究中的应用

Research on cloning gene associated with lung cancer metastasis using suppression subtractive hybridization

为探讨肿瘤转移发生的分子生物学机制奠定基础 ,通过使用抑制消减杂交技术从一对同一亲本、转移表型不同的人肺巨细胞癌细胞株中分离转移抑制相关基因或核苷酸片段。结果获得 5个在低转移肺巨细胞癌中高表达的、均与已知的人类基因片段有很高同源性的核苷酸片段 ,它们可能在维持肿瘤细胞自身稳定。

In order to provide important information for early diagnosis and therapy of tumor metastasis, a study on the biological regulation of metastasis at molecular level was carried out by isolation and identification of human metastatic suppressor gene or related DNA sequence′s from a pair of cell clones with different metastatic phenotype. Suppression subtractive hybridization technique(SSH),gene cloning and analysis using GenBank database were applied to this investigation. Two cell clones of lung giant cell carcinoma originated from same patient were used. PLA?801C cell clone showed lower metastatic potentiality was used as tester, while PLA?801D as a driver. Five obtained cDNA clones were expressed at much higher levels in the poorly metastasis cancer cell clone than the highly metastatic one. Homology analysis of all five cDNA fragments by BLASTN through GenBank showed high homologous with known human genes. Using SSH, we have identified five cDNA fragments which were expressed at much higher levels in the poorly metastasis cancer cell clone than the highly metastatic one. Therefore, it is supposed that they might play important roes in metastatic suppression of tumor cells and have potential application in diagnosis and treatment of metastasis.