摘要
用纯化的CTB后腿肌肉注射免疫小鼠 ,每次 12 .5 μg ,每隔 14天加强一次 ,共注射三次 ,末次免疫后两周收集血清 ,免疫小鼠诱导产生了特异性抗CTB抗体。在CHO细胞中 ,免疫小鼠血清能够中和CT的细胞病变效应 ;小鼠肠结扎实验表明 ,抗CTB血清能够抑制CT结合细胞表面受体GM1神经节苷脂。
Plant-derived CTB was used to immunize mice, and the serum samples were examined for CTB-specific antibodies. It indicated that the immunized mice produced significantly high titer of anti-CTB serum. The cytopathic effect of CT on CHO cell was neutralized by the serum, and the protective efficacy of immunization was assayed by mouse ileal loop ligation. All these results demonstrated that the antiserum showed protection against a bacterial enterotoxin.
出处
《生物技术通讯》
CAS
2000年第3期192-195,共4页
Letters in Biotechnology
