抗虫的转AaIT基因杨树的获得

Insect resistant Transgenic Poplar Expressing AaIT Gene

通过根癌农杆菌叶盘法将构建在双元载体上的昆虫特异性神经蝎毒素AaIT基因转化至中国南方杨树N106(小叶杨×美洲黑杨,P.deltoides×P.simonii)中,共获得了62株再生植株。PCR分析及PCR产物Southernblotting的分析结果表明,AaIT基因整合在再生植株的基因组上。对部分转AaIT基因植株进行了杀虫实验,转基因植株A5对一龄舞毒蛾(Lymantriadispar)幼虫有明显的抗性,饲喂转基因杨树叶片的幼虫死亡率显著高于未转基因对照植株,其取食面积小,存活幼虫体重明显小于对照。ELISA分析证明了AaIT蛋白的表达。

Insect specific scorpion neurotoxin AaIT gene inserted into a binary vector was transferred into a hybrid poplar clone N 106( P.deltoides×P.simonii ) growing in the Southern of China.We obtained sixty two regenerated plants by Agrobacterium tumefaciens transferring system.PCR and PCR Southern analysis showed that AaIT gene was incorporated into the genome of some recovered poplar plants.One of the transformed plants named A5 was significantly resistant to feeding by first instar larvae of Lymantria dispar, compared with the untransformed control plant.It caused a decrease in leaf consumption by larvae,a lower larval weight gain and a higher larval motality rate of Lymantria dispar. ELISA analysis proved that AaIT gene was expressed in this transfomed poplar plant.