柑桔细胞电融合再生两个种间体细胞杂种

Two Interspecific Somatic Hybrid Plants Regenerated Via Protoplast Electro fusion

朋娜脐橙(CitrussinensisOsbeck)胚性细胞悬浮系原生质体分别与粗柠檬(C.jambhiriLush)、枸头橙(C.aurantium)叶肉原生质体经电场诱导而融合。经培养,两组合均获得再生植株。对朋娜脐橙+粗柠檬的再生胚状体进行染色体计数,随机取样的52个胚状体中,26个为四倍体,另外26个为二倍体;对74棵再生植株进行染色体计数,由此说明都为四倍体;表明体细胞杂种在植株再生过程中具有明显的竞争优势。朋娜脐橙+枸头橙再生的14棵植株都为四倍体。对朋娜脐橙+粗柠檬部分植株进行POX同工酶和RAPD分析,表明所有检测植株都为杂种。朋娜脐橙+枸头橙再生植株经RAPD分析,表明也为杂种。

Protoplasts isolated from cell suspension cultures of ‘Bonnaza’ navel orange ( Citrus sinensis L.Osbeck) were electrically fused with mesophyll protoplasts of rough lemon ( Citrus jambhiri Lush)and Goutou orange ( Citrus aurantium L.)respectively.Plants regenerated from both fusion combinations.Chromosome counting of randomly selected fifty two globular embryoids as well as all the regenerated seventy four plants from Bonnaza navel +rough lemon revealed that twenty six embryoids were tetraploids,and the rest were diploids while 100% regenerated plants were tetraploids.The results inferred that somatic hybrids were more competitive than parental genotypes in the process of plant regeneration.All the regenerated 14 plants from Bonnaza navel +Goutou orange were tetraploids as revealed by chromosome counting.POX isozyme and RAPD analysis verified that the plants from Bonnaza navel+rough lemon were hybrids,and RAPD analysis confirmed the hybridity of those from Bonnaza navel+Goutou orange.